In den in vitro Tests wurden die Mikroorganismen auf ihre antagonistische Wirkung in Dualkulturen auf Kartoffel-Dextrose-Agar geprüft. Es wurde die antagonische Wirkung von Trichoderma harzianum und Gliocladium roseum auf das Wachstum von Septoria tritici in vitro und die Befallsstärke an im Gewächshaus kultivierten Weizenpflanzen ausgewertet. Further studies are needed before unequivocal conclusions can be drawn. However, there has been a tendency to reduce the percentage of necrotic area with pyenidial coverage when compared with the control. The in vivo inhibitory capacity of the two antagonists assayed were not expressed on the wheat plants tested. Although, the further stomatic penetration of S. The spore germination and the superficial mycelial development of S. tritici colony was observed with the bioassay on wheat leaves. tritici colony and covered it completely. Both antagonistic agents grew over the S. A histopathological clarification and staining technique was used in order to observe, if the penetration and development of the pathogen on wheat leaves was modified in the presence of the antagonistic agents. For the in vitro tests, the micro-organisms were confronted through dual cultures on PDA. The suitability of Trichoderma harzianum and Gliocladium roseum as biocontrol agents on the in vitro growing of Septoria tritici and their efficiency in reducing disease severity on wheat plants was evaluated under greenhouse conditions.
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